Interleukin-1 exerts distinct actions on different cell types of the brain in vitro

Interleukin-1 exerts distinct actions on different cell types of the brain in vitro

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Ying An, Qun Chen, Ning QuanDepartment Fridge Roller of Oral Biology, Ohio State University, Columbus, OH, USAAbstract: Interleukin-1 (IL-1) is a critical neuroinflammatory mediator in the central nervous system (CNS).In this study, we investigated the effect of IL-1 on inducing inflammation-related gene expression in three astrocyte, two microglial, and one brain endothelial cell line.Interleukin-1 beta (IL-1β) is found to be produced by the two microglial cell lines constitutively, but these cells do not respond to IL-1β stimulation.

The three astrocyte cell lines responded to IL-1ß stimulation by expressing MCP-1, CXCL-1, and VCAM-1, but different subtypes of astrocytes exhibited different expression profiles after IL-1β stimulation.The brain endothelial cells showed strongest response to IL-1β by producing MCP-1, CXCL-1, VCAM-1, ICAM-1, IL-6, and COX-2 mRNA.The induction of endothelial COX-2 mRNA Lower Console Keyboard is shown to be mediated by p38 MAPK pathway, whereas the induction of other genes is mediated by the NF-κB pathway.

These results demonstrate that IL-1 exerts distinct cell type-specific action in CNS cells and suggest that IL-1-mediated neuroinflammation is the result of the summation of multiple responses from different cell types in the CNS to IL-1.Keywords: astrocyte, microglia, endothelial cells, signal transduction pathways, gene expression .